                         Listado de secuencias

<110>  UNIVERSIDAD DE ANTOFAGASTA
 
<120>  ENZIMA MODIFICADA CON ACTIVIDAD ALFA-AMILASA CON EXTREMA 
       TERMOTOLERANCIA Y TERMOESTABILIDAD, METODO DE OBTENCIN DE LA 
       ENZIMA Y SU USO PARA LA INDUSTRIA DEL PROCESAMIENTO DEL ALMIDN

<130>  PAT 3143/2013

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  1518
<212>  DNA
<213>  Anoxybacillus sp

<400>  1
atgaaacgtg tatttcgcgc gctacttgtt tttattttgt tgttgtctgt gacgacgcca       60

gcgagtgcaa aaacggagcg aacgtggcaa gatgaacgca tttattttat tatggtcgac      120

cgttttaata acgggaatcc gaaaaacgat gacgatgtaa atgtgcatga tccgaaagcg      180

tatcatggcg gcgatttaca agggattatt gacaagctcg actacataaa aggaatgggt      240

tttacagcca tttggctgac gccgattttt gcgaacgaaa aaggcgggta tcacggctat      300

tggattgaag acttttataa agtagaagag cattttggaa cgcttgatga cttcaaacgg      360

cttgtgaaag aagcgcataa acgcgatatg aaagtcattt tagatttcgt cgtcaaccat      420

acaggttaca atcatccgtg gttgaatgat ccagcgaaaa aagattggtt tcatgagaaa      480

aaagacattt ttaactgggc gaaccagcaa gaagtagaaa acggttggtt gttcggcctc      540

ccggatttag cgcaagaaaa tccggaagtg aaagcgtatt tattcgatgt cgcgaaatgg      600

tggattaaag aaacagacgt tgacggctat cgtttagata cggtgaaaca tgtgccaaaa      660

tcgttttggg acgagttttc aaaagaagtg aagtcggtga aacaagactt tttccttctt      720

ggagaagtat ggcatgacga cccgcgttat gtcgcggaat atgggaagca cggcatcgat      780

gcgctaattg attttccgtt ttacaaagaa gcgtcaacca ttttttcaaa cgtcgatcaa      840

tcgcttgagc cgctttacaa cgtatggaag cgaaatgtgg cgttttatga ccgaccgtat      900

ttgctcggaa cgtttttaga caatcacgat accgttcggt ttacgcgttt agctttgcaa      960

aatcgcatca atccggtgac gcgtttaaag ctcggattga cgtatttgtt tgcagcgccg     1020

ggcgttccaa tcatgtacta tggaacagaa attgcgcttg acggcgggga agatccagat     1080

aaccgccgtt taatgaattt ccgtaccgat aaagagttga ttgattatgt gacaaagctc     1140

ggggaattgc gcgaaaaact tccgtcgctt cgtcgcggtg attttgaatt gctttatgaa     1200

aaagacggca tggccctatt taagcggacg tatgaaaagg aaacgacggt aattgcgatc     1260

aataatacgt caaaaacgca aaaagtgacg ctcgacgatg agctagaaca aggaaaagaa     1320

ttgcgcgggt tgctggcggg agatttagtg cgcagcaaag acggcaagta cgacatcatt     1380

ttagatcgcg aaacggcgga aatttacgtg cttgcgccaa aaacgggatt aaacatacca     1440

tttattgcgg cgcttgtggc agtatatacg gcatttggtc tgtttttata cttcgcgcgt     1500

aaacgaaaag catcatga                                                   1518


<210>  2
<211>  1433
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Secuencia nucleotdica de alfa-amilasa modificada 
       sin dominio transmembrana

<220>
<221>  misc_feature
<222>  (70)..(70)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (87)..(87)
<223>  n is a, c, g, or t

<400>  2
atgaaacgtg tatttcgcgc gctacttgtt tttattttgt tgttgtctgt gacgacgcca       60

gcgagtgcan aaaacggagc gaacgtnggc aagatgaacg catttatttt attatggtcg      120

accgttttaa taacgggaat ccgaaaaacg atgacgatgt aaatgtgcat gatccgaaag      180

cgtatcatgg cggcgattta caagggatta ttgacaagct cgactacata aaaggaatgg      240

gttttacagc catttggctg acgccgattt ttgcgaacga aaaaggcggg tatcacggct      300

attggattga agacttttat aaagtagaag agcattttgg aacgcttgat gacttcaaac      360

ggcttgtgaa agaagcgcat aaacgcgata tgaaagtcat tttagatttc gtcgtcaacc      420

atacaggtta caatcatccg tggttgaatg atccagcgaa aaaagattgg tttcatgaga      480

aaaaagacat ttttaactgg gcgaaccagc aagaagtaga aaacggttgg ttgttcggcc      540

tcccggattt agcgcaagaa aatccggaag tgaaagcgta tttattcgat gtcgcgaaat      600

ggtggattaa agaaacagac gttgacggct atcgtttaga tacggtgaaa catgtgccaa      660

aatcgttttg ggacgagttt tcaaaagaag tgaagtcggt gaaacaagac tttttccttc      720

ttggagaagt atggcatgac gacccgcgtt atgtcgcgga atatgggaag cacggcatcg      780

atgcgctaat tgattttccg ttttacaaag aagcgtcaac cattttttca aacgtcgatc      840

aatcgcttga gccgctttac aacgtatgga agcgaaatgt ggcgttttat gaccgaccgt      900

atttgctcgg aacgttttta gacaatcacg ataccgttcg gtttacgcgt ttagctttgc      960

aaaatcgcat caatccggtg acgcgtttaa agctcggatt gacgtatttg tttgcagcgc     1020

cgggcgttcc aatcatgtac tatggaacag aaattgcgct tgacggcggg gaagatccag     1080

ataaccgccg tttaatgaat ttccgtaccg ataaagagtt gattgattat gtgacaaagc     1140

tcggggaatt gcgcgaaaaa cttccgtcgc ttcgtcgcgg tgattttgaa ttgctttatg     1200

aaaaagacgg catggcccta tttaagcgga cgtatgaaaa ggaaacgacg gtaattgcga     1260

tcaataatac gtcaaaaacg caaaaagtga cgctcgacga tgagctagaa caaggaaaag     1320

aattgcgcgg gttgctggcg ggagatttag tgcgcagcaa agacggcaag tacgacatca     1380

ttttagatcg cgaaacggcg gaaatttacg tgcttgcgcc aaaaacggga tta            1433


<210>  3
<211>  1451
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Secuencia nucleotdica de alfa-amilasa modificada 
       sin dominio pptido seal

<220>
<221>  misc_feature
<222>  (1346)..(1346)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (1364)..(1364)
<223>  n is a, c, g, or t

<400>  3
aaaacggagc gaacgtggca agatgaacgc atttatttta ttatggtcga ccgttttaat       60

aacgggaatc cgaaaaacga tgacgatgta aatgtgcatg atccgaaagc gtatcatggc      120

ggcgatttac aagggattat tgacaagctc gactacataa aaggaatggg ttttacagcc      180

atttggctga cgccgatttt tgcgaacgaa aaaggcgggt atcacggcta ttggattgaa      240

gacttttata aagtagaaga gcattttgga acgcttgatg acttcaaacg gcttgtgaaa      300

gaagcgcata aacgcgatat gaaagtcatt ttagatttcg tcgtcaacca tacaggttac      360

aatcatccgt ggttgaatga tccagcgaaa aaagattggt ttcatgagaa aaaagacatt      420

tttaactggg cgaaccagca agaagtagaa aacggttggt tgttcggcct cccggattta      480

gcgcaagaaa atccggaagt gaaagcgtat ttattcgatg tcgcgaaatg gtggattaaa      540

gaaacagacg ttgacggcta tcgtttagat acggtgaaac atgtgccaaa atcgttttgg      600

gacgagtttt caaaagaagt gaagtcggtg aaacaagact ttttccttct tggagaagta      660

tggcatgacg acccgcgtta tgtcgcggaa tatgggaagc acggcatcga tgcgctaatt      720

gattttccgt tttacaaaga agcgtcaacc attttttcaa acgtcgatca atcgcttgag      780

ccgctttaca acgtatggaa gcgaaatgtg gcgttttatg accgaccgta tttgctcgga      840

acgtttttag acaatcacga taccgttcgg tttacgcgtt tagctttgca aaatcgcatc      900

aatccggtga cgcgtttaaa gctcggattg acgtatttgt ttgcagcgcc gggcgttcca      960

atcatgtact atggaacaga aattgcgctt gacggcgggg aagatccaga taaccgccgt     1020

ttaatgaatt tccgtaccga taaagagttg attgattatg tgacaaagct cggggaattg     1080

cgcgaaaaac ttccgtcgct tcgtcgcggt gattttgaat tgctttatga aaaagacggc     1140

atggccctat ttaagcggac gtatgaaaag gaaacgacgg taattgcgat caataatacg     1200

tcaaaaacgc aaaaagtgac gctcgacgat gagctagaac aaggaaaaga attgcgcggg     1260

ttgctggcgg gagatttagt gcgcagcaaa gacggcaagt acgacatcat tttagatcgc     1320

gaaacggcgg aaatttacgt gcttgncgcc aaaaacggga ttanaacata ccatttattg     1380

cggcgcttgt ggcagtatat acggcatttg gtctgttttt atacttcgcg cgtaaacgaa     1440

aagcatcatg a                                                          1451


<210>  4
<211>  1362
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Secuencia nucleotdica de alfa-amilasa modificada 
       sin dominios transmembrada y peptido seal

<400>  4
aaaacggagc gaacgtggca agatgaacgc atttatttta ttatggtcga ccgttttaat       60

aacgggaatc cgaaaaacga tgacgatgta aatgtgcatg atccgaaagc gtatcatggc      120

ggcgatttac aagggattat tgacaagctc gactacataa aaggaatggg ttttacagcc      180

atttggctga cgccgatttt tgcgaacgaa aaaggcgggt atcacggcta ttggattgaa      240

gacttttata aagtagaaga gcattttgga acgcttgatg acttcaaacg gcttgtgaaa      300

gaagcgcata aacgcgatat gaaagtcatt ttagatttcg tcgtcaacca tacaggttac      360

aatcatccgt ggttgaatga tccagcgaaa aaagattggt ttcatgagaa aaaagacatt      420

tttaactggg cgaaccagca agaagtagaa aacggttggt tgttcggcct cccggattta      480

gcgcaagaaa atccggaagt gaaagcgtat ttattcgatg tcgcgaaatg gtggattaaa      540

gaaacagacg ttgacggcta tcgtttagat acggtgaaac atgtgccaaa atcgttttgg      600

gacgagtttt caaaagaagt gaagtcggtg aaacaagact ttttccttct tggagaagta      660

tggcatgacg acccgcgtta tgtcgcggaa tatgggaagc acggcatcga tgcgctaatt      720

gattttccgt tttacaaaga agcgtcaacc attttttcaa acgtcgatca atcgcttgag      780

ccgctttaca acgtatggaa gcgaaatgtg gcgttttatg accgaccgta tttgctcgga      840

acgtttttag acaatcacga taccgttcgg tttacgcgtt tagctttgca aaatcgcatc      900

aatccggtga cgcgtttaaa gctcggattg acgtatttgt ttgcagcgcc gggcgttcca      960

atcatgtact atggaacaga aattgcgctt gacggcgggg aagatccaga taaccgccgt     1020

ttaatgaatt tccgtaccga taaagagttg attgattatg tgacaaagct cggggaattg     1080

cgcgaaaaac ttccgtcgct tcgtcgcggt gattttgaat tgctttatga aaaagacggc     1140

atggccctat ttaagcggac gtatgaaaag gaaacgacgg taattgcgat caataatacg     1200

tcaaaaacgc aaaaagtgac gctcgacgat gagctagaac aaggaaaaga attgcgcggg     1260

ttgctggcgg gagatttagt gcgcagcaaa gacggcaagt acgacatcat tttagatcgc     1320

gaaacggcgg aaatttacgt gcttgcgcca aaaacgggat ta                        1362


<210>  5
<211>  505
<212>  PRT
<213>  Anoxybacillus sp

<400>  5

Met Lys Arg Val Phe Arg Ala Leu Leu Val Phe Ile Leu Leu Leu Ser 
1               5                   10                  15      


Val Thr Thr Pro Ala Ser Ala Lys Thr Glu Arg Thr Trp Gln Asp Glu 
            20                  25                  30          


Arg Ile Tyr Phe Ile Met Val Asp Arg Phe Asn Asn Gly Asn Pro Lys 
        35                  40                  45              


Asn Asp Asp Asp Val Asn Val His Asp Pro Lys Ala Tyr His Gly Gly 
    50                  55                  60                  


Asp Leu Gln Gly Ile Ile Asp Lys Leu Asp Tyr Ile Lys Gly Met Gly 
65                  70                  75                  80  


Phe Thr Ala Ile Trp Leu Thr Pro Ile Phe Ala Asn Glu Lys Gly Gly 
                85                  90                  95      


Tyr His Gly Tyr Trp Ile Glu Asp Phe Tyr Lys Val Glu Glu His Phe 
            100                 105                 110         


Gly Thr Leu Asp Asp Phe Lys Arg Leu Val Lys Glu Ala His Lys Arg 
        115                 120                 125             


Asp Met Lys Val Ile Leu Asp Phe Val Val Asn His Thr Gly Tyr Asn 
    130                 135                 140                 


His Pro Trp Leu Asn Asp Pro Ala Lys Lys Asp Trp Phe His Glu Lys 
145                 150                 155                 160 


Lys Asp Ile Phe Asn Trp Ala Asn Gln Gln Glu Val Glu Asn Gly Trp 
                165                 170                 175     


Leu Phe Gly Leu Pro Asp Leu Ala Gln Glu Asn Pro Glu Val Lys Ala 
            180                 185                 190         


Tyr Leu Phe Asp Val Ala Lys Trp Trp Ile Lys Glu Thr Asp Val Asp 
        195                 200                 205             


Gly Tyr Arg Leu Asp Thr Val Lys His Val Pro Lys Ser Phe Trp Asp 
    210                 215                 220                 


Glu Phe Ser Lys Glu Val Lys Ser Val Lys Gln Asp Phe Phe Leu Leu 
225                 230                 235                 240 


Gly Glu Val Trp His Asp Asp Pro Arg Tyr Val Ala Glu Tyr Gly Lys 
                245                 250                 255     


His Gly Ile Asp Ala Leu Ile Asp Phe Pro Phe Tyr Lys Glu Ala Ser 
            260                 265                 270         


Thr Ile Phe Ser Asn Val Asp Gln Ser Leu Glu Pro Leu Tyr Asn Val 
        275                 280                 285             


Trp Lys Arg Asn Val Ala Phe Tyr Asp Arg Pro Tyr Leu Leu Gly Thr 
    290                 295                 300                 


Phe Leu Asp Asn His Asp Thr Val Arg Phe Thr Arg Leu Ala Leu Gln 
305                 310                 315                 320 


Asn Arg Ile Asn Pro Val Thr Arg Leu Lys Leu Gly Leu Thr Tyr Leu 
                325                 330                 335     


Phe Ala Ala Pro Gly Val Pro Ile Met Tyr Tyr Gly Thr Glu Ile Ala 
            340                 345                 350         


Leu Asp Gly Gly Glu Asp Pro Asp Asn Arg Arg Leu Met Asn Phe Arg 
        355                 360                 365             


Thr Asp Lys Glu Leu Ile Asp Tyr Val Thr Lys Leu Gly Glu Leu Arg 
    370                 375                 380                 


Glu Lys Leu Pro Ser Leu Arg Arg Gly Asp Phe Glu Leu Leu Tyr Glu 
385                 390                 395                 400 


Lys Asp Gly Met Ala Leu Phe Lys Arg Thr Tyr Glu Lys Glu Thr Thr 
                405                 410                 415     


Val Ile Ala Ile Asn Asn Thr Ser Lys Thr Gln Lys Val Thr Leu Asp 
            420                 425                 430         


Asp Glu Leu Glu Gln Gly Lys Glu Leu Arg Gly Leu Leu Ala Gly Asp 
        435                 440                 445             


Leu Val Arg Ser Lys Asp Gly Lys Tyr Asp Ile Ile Leu Asp Arg Glu 
    450                 455                 460                 


Thr Ala Glu Ile Tyr Val Leu Ala Pro Lys Thr Gly Leu Asn Ile Pro 
465                 470                 475                 480 


Phe Ile Ala Ala Leu Val Ala Val Tyr Thr Ala Phe Gly Leu Phe Leu 
                485                 490                 495     


Tyr Phe Ala Arg Lys Arg Lys Ala Ser 
            500                 505 


<210>  6
<211>  477
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Secuencia aminoacdica de alfa-amilasa modificada 
       sin dominio transmembrana

<400>  6

Met Lys Arg Val Phe Arg Ala Leu Leu Val Phe Ile Leu Leu Leu Ser 
1               5                   10                  15      


Val Thr Thr Pro Ala Ser Ala Lys Thr Glu Arg Thr Trp Gln Asp Glu 
            20                  25                  30          


Arg Ile Tyr Phe Ile Met Val Asp Arg Phe Asn Asn Gly Asn Pro Lys 
        35                  40                  45              


Asn Asp Asp Asp Val Asn Val His Asp Pro Lys Ala Tyr His Gly Gly 
    50                  55                  60                  


Asp Leu Gln Gly Ile Ile Asp Lys Leu Asp Tyr Ile Lys Gly Met Gly 
65                  70                  75                  80  


Phe Thr Ala Ile Trp Leu Thr Pro Ile Phe Ala Asn Glu Lys Gly Gly 
                85                  90                  95      


Tyr His Gly Tyr Trp Ile Glu Asp Phe Tyr Lys Val Glu Glu His Phe 
            100                 105                 110         


Gly Thr Leu Asp Asp Phe Lys Arg Leu Val Lys Glu Ala His Lys Arg 
        115                 120                 125             


Asp Met Lys Val Ile Leu Asp Phe Val Val Asn His Thr Gly Tyr Asn 
    130                 135                 140                 


His Pro Trp Leu Asn Asp Pro Ala Lys Lys Asp Trp Phe His Glu Lys 
145                 150                 155                 160 


Lys Asp Ile Phe Asn Trp Ala Asn Gln Gln Glu Val Glu Asn Gly Trp 
                165                 170                 175     


Leu Phe Gly Leu Pro Asp Leu Ala Gln Glu Asn Pro Glu Val Lys Ala 
            180                 185                 190         


Tyr Leu Phe Asp Val Ala Lys Trp Trp Ile Lys Glu Thr Asp Val Asp 
        195                 200                 205             


Gly Tyr Arg Leu Asp Thr Val Lys His Val Pro Lys Ser Phe Trp Asp 
    210                 215                 220                 


Glu Phe Ser Lys Glu Val Lys Ser Val Lys Gln Asp Phe Phe Leu Leu 
225                 230                 235                 240 


Gly Glu Val Trp His Asp Asp Pro Arg Tyr Val Ala Glu Tyr Gly Lys 
                245                 250                 255     


His Gly Ile Asp Ala Leu Ile Asp Phe Pro Phe Tyr Lys Glu Ala Ser 
            260                 265                 270         


Thr Ile Phe Ser Asn Val Asp Gln Ser Leu Glu Pro Leu Tyr Asn Val 
        275                 280                 285             


Trp Lys Arg Asn Val Ala Phe Tyr Asp Arg Pro Tyr Leu Leu Gly Thr 
    290                 295                 300                 


Phe Leu Asp Asn His Asp Thr Val Arg Phe Thr Arg Leu Ala Leu Gln 
305                 310                 315                 320 


Asn Arg Ile Asn Pro Val Thr Arg Leu Lys Leu Gly Leu Thr Tyr Leu 
                325                 330                 335     


Phe Ala Ala Pro Gly Val Pro Ile Met Tyr Tyr Gly Thr Glu Ile Ala 
            340                 345                 350         


Leu Asp Gly Gly Glu Asp Pro Asp Asn Arg Arg Leu Met Asn Phe Arg 
        355                 360                 365             


Thr Asp Lys Glu Leu Ile Asp Tyr Val Thr Lys Leu Gly Glu Leu Arg 
    370                 375                 380                 


Glu Lys Leu Pro Ser Leu Arg Arg Gly Asp Phe Glu Leu Leu Tyr Glu 
385                 390                 395                 400 


Lys Asp Gly Met Ala Leu Phe Lys Arg Thr Tyr Glu Lys Glu Thr Thr 
                405                 410                 415     


Val Ile Ala Ile Asn Asn Thr Ser Lys Thr Gln Lys Val Thr Leu Asp 
            420                 425                 430         


Asp Glu Leu Glu Gln Gly Lys Glu Leu Arg Gly Leu Leu Ala Gly Asp 
        435                 440                 445             


Leu Val Arg Ser Lys Asp Gly Lys Tyr Asp Ile Ile Leu Asp Arg Glu 
    450                 455                 460                 


Thr Ala Glu Ile Tyr Val Leu Ala Pro Lys Thr Gly Leu 
465                 470                 475         


<210>  7
<211>  483
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Secuencia aminoacdica de alfa-amilasa modificada 
       sin dominio pptido seal

<400>  7

Met Lys Thr Glu Arg Thr Trp Gln Asp Glu Arg Ile Tyr Phe Ile Met 
1               5                   10                  15      


Val Asp Arg Phe Asn Asn Gly Asn Pro Lys Asn Asp Asp Asp Val Asn 
            20                  25                  30          


Val His Asp Pro Lys Ala Tyr His Gly Gly Asp Leu Gln Gly Ile Ile 
        35                  40                  45              


Asp Lys Leu Asp Tyr Ile Lys Gly Met Gly Phe Thr Ala Ile Trp Leu 
    50                  55                  60                  


Thr Pro Ile Phe Ala Asn Glu Lys Gly Gly Tyr His Gly Tyr Trp Ile 
65                  70                  75                  80  


Glu Asp Phe Tyr Lys Val Glu Glu His Phe Gly Thr Leu Asp Asp Phe 
                85                  90                  95      


Lys Arg Leu Val Lys Glu Ala His Lys Arg Asp Met Lys Val Ile Leu 
            100                 105                 110         


Asp Phe Val Val Asn His Thr Gly Tyr Asn His Pro Trp Leu Asn Asp 
        115                 120                 125             


Pro Ala Lys Lys Asp Trp Phe His Glu Lys Lys Asp Ile Phe Asn Trp 
    130                 135                 140                 


Ala Asn Gln Gln Glu Val Glu Asn Gly Trp Leu Phe Gly Leu Pro Asp 
145                 150                 155                 160 


Leu Ala Gln Glu Asn Pro Glu Val Lys Ala Tyr Leu Phe Asp Val Ala 
                165                 170                 175     


Lys Trp Trp Ile Lys Glu Thr Asp Val Asp Gly Tyr Arg Leu Asp Thr 
            180                 185                 190         


Val Lys His Val Pro Lys Ser Phe Trp Asp Glu Phe Ser Lys Glu Val 
        195                 200                 205             


Lys Ser Val Lys Gln Asp Phe Phe Leu Leu Gly Glu Val Trp His Asp 
    210                 215                 220                 


Asp Pro Arg Tyr Val Ala Glu Tyr Gly Lys His Gly Ile Asp Ala Leu 
225                 230                 235                 240 


Ile Asp Phe Pro Phe Tyr Lys Glu Ala Ser Thr Ile Phe Ser Asn Val 
                245                 250                 255     


Asp Gln Ser Leu Glu Pro Leu Tyr Asn Val Trp Lys Arg Asn Val Ala 
            260                 265                 270         


Phe Tyr Asp Arg Pro Tyr Leu Leu Gly Thr Phe Leu Asp Asn His Asp 
        275                 280                 285             


Thr Val Arg Phe Thr Arg Leu Ala Leu Gln Asn Arg Ile Asn Pro Val 
    290                 295                 300                 


Thr Arg Leu Lys Leu Gly Leu Thr Tyr Leu Phe Ala Ala Pro Gly Val 
305                 310                 315                 320 


Pro Ile Met Tyr Tyr Gly Thr Glu Ile Ala Leu Asp Gly Gly Glu Asp 
                325                 330                 335     


Pro Asp Asn Arg Arg Leu Met Asn Phe Arg Thr Asp Lys Glu Leu Ile 
            340                 345                 350         


Asp Tyr Val Thr Lys Leu Gly Glu Leu Arg Glu Lys Leu Pro Ser Leu 
        355                 360                 365             


Arg Arg Gly Asp Phe Glu Leu Leu Tyr Glu Lys Asp Gly Met Ala Leu 
    370                 375                 380                 


Phe Lys Arg Thr Tyr Glu Lys Glu Thr Thr Val Ile Ala Ile Asn Asn 
385                 390                 395                 400 


Thr Ser Lys Thr Gln Lys Val Thr Leu Asp Asp Glu Leu Glu Gln Gly 
                405                 410                 415     


Lys Glu Leu Arg Gly Leu Leu Ala Gly Asp Leu Val Arg Ser Lys Asp 
            420                 425                 430         


Gly Lys Tyr Asp Ile Ile Leu Asp Arg Glu Thr Ala Glu Ile Tyr Val 
        435                 440                 445             


Leu Ala Pro Lys Thr Gly Leu Asn Ile Pro Phe Ile Ala Ala Leu Val 
    450                 455                 460                 


Ala Val Tyr Thr Ala Phe Gly Leu Phe Leu Tyr Phe Ala Arg Lys Arg 
465                 470                 475                 480 


Lys Ala Ser 
            


<210>  8
<211>  455
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Secuencia aminoacdica de alfa-amilasa modificada 
       sin dominios transmembrada y peptido seal

<400>  8

Met Lys Thr Glu Arg Thr Trp Gln Asp Glu Arg Ile Tyr Phe Ile Met 
1               5                   10                  15      


Val Asp Arg Phe Asn Asn Gly Asn Pro Lys Asn Asp Asp Asp Val Asn 
            20                  25                  30          


Val His Asp Pro Lys Ala Tyr His Gly Gly Asp Leu Gln Gly Ile Ile 
        35                  40                  45              


Asp Lys Leu Asp Tyr Ile Lys Gly Met Gly Phe Thr Ala Ile Trp Leu 
    50                  55                  60                  


Thr Pro Ile Phe Ala Asn Glu Lys Gly Gly Tyr His Gly Tyr Trp Ile 
65                  70                  75                  80  


Glu Asp Phe Tyr Lys Val Glu Glu His Phe Gly Thr Leu Asp Asp Phe 
                85                  90                  95      


Lys Arg Leu Val Lys Glu Ala His Lys Arg Asp Met Lys Val Ile Leu 
            100                 105                 110         


Asp Phe Val Val Asn His Thr Gly Tyr Asn His Pro Trp Leu Asn Asp 
        115                 120                 125             


Pro Ala Lys Lys Asp Trp Phe His Glu Lys Lys Asp Ile Phe Asn Trp 
    130                 135                 140                 


Ala Asn Gln Gln Glu Val Glu Asn Gly Trp Leu Phe Gly Leu Pro Asp 
145                 150                 155                 160 


Leu Ala Gln Glu Asn Pro Glu Val Lys Ala Tyr Leu Phe Asp Val Ala 
                165                 170                 175     


Lys Trp Trp Ile Lys Glu Thr Asp Val Asp Gly Tyr Arg Leu Asp Thr 
            180                 185                 190         


Val Lys His Val Pro Lys Ser Phe Trp Asp Glu Phe Ser Lys Glu Val 
        195                 200                 205             


Lys Ser Val Lys Gln Asp Phe Phe Leu Leu Gly Glu Val Trp His Asp 
    210                 215                 220                 


Asp Pro Arg Tyr Val Ala Glu Tyr Gly Lys His Gly Ile Asp Ala Leu 
225                 230                 235                 240 


Ile Asp Phe Pro Phe Tyr Lys Glu Ala Ser Thr Ile Phe Ser Asn Val 
                245                 250                 255     


Asp Gln Ser Leu Glu Pro Leu Tyr Asn Val Trp Lys Arg Asn Val Ala 
            260                 265                 270         


Phe Tyr Asp Arg Pro Tyr Leu Leu Gly Thr Phe Leu Asp Asn His Asp 
        275                 280                 285             


Thr Val Arg Phe Thr Arg Leu Ala Leu Gln Asn Arg Ile Asn Pro Val 
    290                 295                 300                 


Thr Arg Leu Lys Leu Gly Leu Thr Tyr Leu Phe Ala Ala Pro Gly Val 
305                 310                 315                 320 


Pro Ile Met Tyr Tyr Gly Thr Glu Ile Ala Leu Asp Gly Gly Glu Asp 
                325                 330                 335     


Pro Asp Asn Arg Arg Leu Met Asn Phe Arg Thr Asp Lys Glu Leu Ile 
            340                 345                 350         


Asp Tyr Val Thr Lys Leu Gly Glu Leu Arg Glu Lys Leu Pro Ser Leu 
        355                 360                 365             


Arg Arg Gly Asp Phe Glu Leu Leu Tyr Glu Lys Asp Gly Met Ala Leu 
    370                 375                 380                 


Phe Lys Arg Thr Tyr Glu Lys Glu Thr Thr Val Ile Ala Ile Asn Asn 
385                 390                 395                 400 


Thr Ser Lys Thr Gln Lys Val Thr Leu Asp Asp Glu Leu Glu Gln Gly 
                405                 410                 415     


Lys Glu Leu Arg Gly Leu Leu Ala Gly Asp Leu Val Arg Ser Lys Asp 
            420                 425                 430         


Gly Lys Tyr Asp Ile Ile Leu Asp Arg Glu Thr Ala Glu Ile Tyr Val 
        435                 440                 445             


Leu Ala Pro Lys Thr Gly Leu 
    450                 455 


<210>  9
<211>  32
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Sinttica - nucletidos de partidor 1

<400>  9
aaggatccat gaaacgtgta tttcgcgcgc ta                                     32


<210>  10
<211>  26
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Sinttica - nucletidos de partidor 2

<400>  10
gtcgactcat gatgcttttc gtttac                                            26


<210>  11
<211>  27
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Sinttica - nucletidos de partidor 3

<400>  11
aaggatccat gaaaacggag cgaacgt                                           27


<210>  12
<211>  26
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Sinttica - nucletidos de partidor 4

<400>  12
gtcgactcat aatcccgttt ttggcg                                            26


